Antibacterial Activity of Silver Nanoparticles Synthesized by Aqueous Extract of Carthamus oxycantha M.Bieb. Against Antibiotics Resistant Bacteria

Authors

Ali Aboud Shareef, Department of Biology, College of Education for Pure Sciences, University of Basrah, Basrah, Iraq.Follow
Zainab Alag Hassan, College of Nursing, University of Basrah, Basrah, Iraq.Follow
Majid Ahmed Kadhim, Al-Sadr Teaching Hospital, Central Laboratory.Follow
Abdulameer Abdullah Al-Mussawi, College of Nursing, University of Basrah, Basrah, Iraq.Follow

Abstract

Antibiotics resistant bacteria have become a global problem as a result of the unprogrammed use of antibiotics, resulting in bacterial strains resistant to many antibiotics, orto all available antibiotics.Plants are a good source of primary and secondary metabolites that have a major role in reducing silver nitrate to silver nanoparticles (AgNPs). The productionof these nanoparticleswere carried out by using aqueous extract of Carthamus oxycantha M.Bieb.This can be verified by colorchangeof the reaction solutionfrom yellow to dark brown because of the excitation of the surface plasmon resonance. AgNPswere characterized byUV-Vis spectroscopy,where they recorded the peak at 420nm.Fourier Transformation-infrared (FTIR) wasconducted to identify the effective plant group that contributesto the formation of AgNPsandit was found that proteins and phenols have the major role in the formation of those nanoparticles. Shapes and sizes of the synthesized AgNPs were characterized byScanning Electron Microscope (SEM)with a range of 50-80nm in size and spherical in shapes. Antibacterial activity of AgNPswere tested against Multi-Drug Resistant bacteria (MDR), Extremely antibiotics Resistant (XDR), and Pan drug-resistant(PAN)bacteria,was done in concentrations ranging from 1000-63 μg/ml. The resultsshowed that there were significant variations between the concentrations, the tested bacteria also showed significant differences in its sensitivity to AgNPs. The results recordeda proportional relation betweenthetype of bacterialresistanceto antibiotics and it's resistant to AgNPs,therefore the most resistant bacteriato AgNPs in this study Enterobacter cloacaeEN2 was resistant to all antibiotics (PAN), while Escherichia coliE11 recorded was the most sensitive bacteria to AgNPs and its resistant only to 3 antibiotics .

Keywords

Antibiotics resistancebacteria, Antibacterial activity, Carthamus oxycantha, Silver nanoparticles.

Article Type

Article

How to Cite this Article

Shareef, Ali Aboud; Hassan, Zainab Alag; Kadhim, Majid Ahmed; and Al-Mussawi, Abdulameer Abdullah (2022) "Antibacterial Activity of Silver Nanoparticles Synthesized by Aqueous Extract of Carthamus oxycantha M.Bieb. Against Antibiotics Resistant Bacteria," Baghdad Science Journal: Vol. 19: Iss. 3, Article 9.
DOI: https://doi.org/10.21123/bsj.2022.19.3.0460