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Abstract

CyanoCobalamin (Vitamin B12) was determined using a simple, reliable, and affordable colorimetric method. According to this method, excess Ferrous sulfate (FeSO4.7H2O, or FS) is used to reduce vitamin B12, and the excess FS reacts with the reagent 1,10-phenanthroline to produce a soluble red color complex. The greatest wavelength of the spectrum of this complex was 510 nm. The concentration linearity range was 5-100 µg/mL. The RSD% values were 0.107-0.259% for intraday and 1.085-0.1378% for interday, while the Rec% values ranged from 95 to 100.833%. LOD and LOQ values were respectively 0.016 µg/mL and 0.053 µg/mL. The method was used to determine the presence of vitamin B12 in medicinal preparations with success.

Keywords

Colorimetric method, CyanoCobalamin, Ferrous Sulfate, 1, 10-phenanthroline, Vitamin B12

Subject Area

Chemistry

First Page

36

Last Page

43

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Receive Date

5-20-2023

Revise Date

11-5-2023

Accept Date

11-7-2023

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