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Abstract

In the present research synthesis and study of biological activity a series of new polymers modified of chitosan with compounds containing azo group. Beginning diazonium salt produced from 3,30-dimethyl-[1,10-biphenyl]-4,40-diamine reacted with concentrated HCl acid and sodium nitrite. The coupling reaction between diazonium salt with substituted aromatic aldehyde to produce Azo derivatives (1-6). Azo Schi bases Chitosan (7-12) were synthesized by condensation of Chitosan with Azo derivatives (1-6) in ethanol with some drops of glacial acetic acid. The structural modifications of Chitosan ring (linked to a bioactive azo moiety) were expected to give new derivatives (7-12) with a diverse range of biological functions. These compounds’ structures have been determined using FT-IR, 1H-NMR spectroscopic and Field Emission Scanning Electron Microscopy studies. Additionally, two other kinds of bacteria: Staphlococcus aureus and E. coli were tested for possible antibacterial properties utilizing some new compounds. Modified Chitosan (7-10) showed high activity comparable to a penicillin (used as the reference antibiotic), Especially the modified polymer(7), which showed high inhibition against both types of bacteria. The anticancer activity of modified chitosan (7) against MCF-7 (human breast carcinoma cells) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine and compare with normal cells WRL-68(the human hepatic cell line). Polymer (7) exhibited a high cancer cell inhibition rate and less toxicity to normal cells.

Keywords

Azo compounds, Chitosan, Diazonium salt, FESEM study, MCF-7, Schiff bases

First Page

428

Last Page

437

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Receive Date

9-14-2023

Revise Date

12-26-2023

Accept Date

12-28-2023

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